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Figure 1. Eugenol inhibited voltage-gated K+ currents in rat trigeminal ganglion neurons. (A) Using pulses from –80 mV to 60 mV for 600 ms with a holding potential of –60 mV, we obtained the representative traces under control, 1 mM eugenol, and wash-out conditions, measured at the points indicated in (B). (B) For time-course recording of voltage-gated K+ currents, we used a depolarizing potential of 20 mV from a holding of –80 mV in 10-second intervals. When trigeminal ganglion neurons were exposed to eugenol (1 mM), the voltage-gated K+ current was readily inhibited in a reversible manner. (C) The dose-response curve of the inhibition of voltage-gated K+ currents by eugenol with the IC50 of 376 ± 90 µM. The number in parentheses represents the number of cells studied.
