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Figure 4. The presence of an implant accelerated osteoblast differentiation. Cellular and molecular analyses at the Ti-6Al-4V (A–C) and 303 SS (D–F) implant surfaces. (A) At post-surgical d5, Trichrome staining indicates the initiation of endosteal bone formation (arrowhead). (B) AP staining corresponds to the areas of new mineralization (arrow), (C) which are undergoing extensive osteoclastic remodeling. (D–F) In situ hybridization on d3 indicates that cells surrounding the implant up-regulate Runx2 and Op; these cells are osteo- rather than chondroprogenitors, because of the absence of Col II expression. Scale bar = 200 microns. (See color version of this Fig. in the online APPENDIX.)
