Click on image to view larger version.
Figure 2. Molecular analyses of healing in the implant bed. Localization of mRNA for collagen type I (Col 1), Osteocalcin (Oc), and Osteopontin (Op) by in situ hybridization on sections adjacent to those shown in Fig. 1. (A–C) By d3, low expression levels for Col 1 are found in the marrow cavity (arrow), while Oc and Op are not expressed. (D–F) By d5, Col 1 is up-regulated in and around the defect within the marrow cavity. Oc mRNA is still not detected. Op-expressing cells are localized near the cortical defect (asterisk). (G–I) By d7, strong expression of all three markers is detected in the defect and in the marrow space underlying the defect. Scale bar = 200 microns. (See color version of this Fig. in the online APPENDIX.)
