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Figure 1. Establishing the time-course and type of healing in the implant bed. A 1.0-mm drill hole was created in the proximal tibiae; samples were then collected at d5, d7, and d28, and stained with Trichrome (TC) and tartrate-resistant acid phosphatase (TRAP). (A–B) At d5, there is no evidence of new bone formation. The injury site is filled with densely packed fibroblasts, (B) while bone debris from the drilling process is engulfed by TRAP-positive osteoclasts. (C–D) By d7, new bone is first detected and undergoes rapid remodeling. (E–F) By d28, woven bone has been replaced by cortical bone fused with the old cortex. Scale bar = 100 microns. (See color version of this Fig. in the online APPENDIX.)
