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Figure 2. Backscattered electron image of lesions of severe fluorosis in the subsurface enamel. Dark areas are hypomineralized. The tissue was plunge-frozen in liquid-nitrogen-cooled Freon 22, freeze-dried at – 80°C, and sputter-coated with carbon for EPMA analysis, and then gold-coated for examination in BEI mode. Scale bar = 100 µm.

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