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Figure 2. Influence of different inhibitors on pSS IgG and RT-PCR analysis. (A) Concentration-response curves of pSS IgG in the absence or in the presence of 5 x 10–6 M OBAA or 1 x 10–4 M aspirin or 4 x 10–8 M rofecoxib. Results are expressed as mean ± SEM of 6 individual pSS IgG samples performed in duplicate. *p < 0.001 vs. pSS IgG alone; **p < 0.005 vs. pSS IgG alone. (B) Effect of pSS IgG on PGE2 production by a rat submandibular gland. Glands were treated with pSS IgG alone or in the presence of calcium-blocking agents (verapamil, 5 x 10–5 M; thansigargin, 1 x 10–6 M) or with a calcium ionophore agent (A 23187, 1 x 10–6 M) alone. Values are mean ± SEM of 6 individual pSS IgG samples performed in duplicate. *p < 0.005 vs. pSS IgG. (C) RT-PCR products of COX-1 and COX-2 obtained from rat submandibular gland alone (basal) or in the presence of 1 x 10–7 M pSS IgG. (D) The densitometry analysis of 7 individual IgG sample tests performed in duplicate. Values are mean ± SEM. Note that the COX-2 band in the presence of pSS IgG increased significantly (p < 0.001) compared with the basal COX-2 band.
