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Figure 3. The effect of buffer modifiers on naproxen accumulation by SCC-25 cells. (Upper panel). The effects of varied (from 0 to 10%) concentrations of ethanol and glycerol on naproxen accumulation by SCC-25 cells. Naproxen accumulation was monitored over 3 min in the presence of the indicated additions to HBSS, under experimental conditions similar to those used in Fig. 1. (Lower panel) The effects of glucosylceramide and Pluronic F-127 (from 0 to 0.1%) on naproxen accumulation by SCC-25 cells. Treatment effects by all 4 modifiers were significant (P < 0.003, repeated-measures ANOVA). Except for 2% ethanol, every individual treatment was significantly different compared with controls (P < 0.05, Holm-Sidak test). The data are presented as the mean ± SEM of 6 experiments.
