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Figure 3. MMP-3 mRNA expression. (a) MMP-3 RT-PCR products in oral and skin fibroblasts relative to ß-actin products for control and TGF-ß-treated conditions. Representative of duplicate experiments for 3 paired oral and skin populations. Appropriate PCR-negative blanks and RT controls were used during all electrophoresis runs (data not shown). (b) Effects of TGF-ß1 and TGF-ß3 on MMP-3 mRNA expression indices. Data are expressed compared with control cultures following normalization of control values for each cell population to 100%. Treatment with both factors, at all concentrations, induced a statistically significant increase in MMP-3 expression (p < 0.05).
