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Figure 3. L929 cell culture nickel sulfate cytotoxicity. L929 cells were cultured (3.5 x 104 cells/100 µL of medium) for 24 hrs (37°C, 5% CO2) to obtain a monolayer, after which different nickel sulfate concentrations were added. After a 48-hour incubation period (37°C, 5% CO2), the cytotoxicity was assessed by crystal violet and MTT assays, as described in MATERIALS & METHODS. The data represent optical density (OD), mean ± SEM (n = 6), obtained at 620 nm (crystal violet assay) and 550 nm (MTT assay). In parentheses are percentages of extract cytotoxicity. Light-microscopic images of the L929-cell monolayer after exposure to different nickel sulfate concentrations are depicted (A,B,C,D) (scale bar: 100 µm) (magnification x 400). *p < 0.05 (medium vs. NiSO4).
