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Figure 3. Cell proliferation rates were measured by immunofluorescent staining of Brdu incorporation, quantitated as relative light units per sec (rlu/s). Fluoride concentrations less than 256 µM promoted ameloblast-lineage cell proliferation, with the peak effect at 16 µM, while the higher levels significantly inhibited cell proliferation relative to control cells. Average (SD) values of groups: 0 µm, 71.9 (19.5); 4 µm, 126.7 (62.5); 16 µm, 164.6 (44.7); 64 µm, 134.3 (48.2); 256 µm, 113.4 (38.9); 1024 µm, 20.5 (12.7); 4096 µm, 14.4 (4.8) (n = 6).
