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Figure 4. The 9.6-kb Dmp1-Cre was highly active in osteocytes in a bone-specific manner. Only positive staining was observed in samples with both ROSA26R and Cre transgenes (left panel), whereas samples that contained the ROSA26R transgene only were negative (right panel). Whole-mount staining from a two-week-old double-transgenic mouse showed blue staining in calvariae (a) and tibia/fibula (b), compared with the control samples containing ROSA26R only. X-Gal-stained frozen sections from the double-transgenic bone showed blue staining in osteocytes in six-day-old (c) and one-month-old calvariae (d) or tibia (e), and in four-month-old (f) mice, compared with their control littermates containing ROSA26R only.
