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Figure 2. The lacZ reporter controlled by the 9.6-kb murine Dmp1 promoter was highly expressed in osteocytes. We previously generated mice in which beta-galactosidase under control of the 9.6-kb murine Dmp1 promoter was shown to be active in odontoblasts and localized in their processes (Lu et al., 2005). Compared with the Dmp1 lacZ knock-in mice, where lacZ expression reflected the endogenous Dmp1 expression (left panel), the expression pattern under control of the 9.6-kb Dmp1 promoter was similar, but with much higher intensity (middle panel), suggesting strong activity in osteocytes. The wild-type control (WT, right panel) showed no lacZ activity. Staining conditions were identical, since all the tibial frozen sections were stained for 2 hrs in X-gal solution at room temperature at the same time.
