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Figure 2. Expressions of TLR1, TLR2, TLR3, TLR4, MD-2, TLR5, TLR6, TLR7, TLR8, TLR9, MyD88, NOD1, and NOD2 in human gingival fibroblasts, detected by flow cytometry. Fibroblasts were cultured until confluent at 37°C. The cell-surface expressions of TLR1, TLR2, TLR4, MD-2, TLR5, TLR6, and intracellular TLR3, TLR7, TLR8, TLR9, MyD88, NOD1, and NOD2 were assessed by flow cytometry. Thin lines represent the isotype Ab control. The results presented are representative of 4 different experiments demonstrating similar results.