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Figure 3. Effects of inhibitors for MAPKs and NF-
B on IL-1
-induced expression of COX-2 mRNA and secretion of PGE2 in odontogenic keratocyst fibroblasts. Fibroblasts (4 x 104 cells/cm2) were incubated with 20 µM PD-98059, 20 µM SB-203580, 40 µM SP-600125, and 20 µM PDTC for 1 hr, and then incubated with 0.1 nM rhIL-1 for 6 hrs (A) or 24 hrs (B) at 37°C. (A) The expression of COX-2 mRNA was measured as described in "MATERIALS & METHODS". (B) The concentration of PGE2 in the culture media was measured as described in "MATERIALS & METHODS". The values were normalized to the concentration of the control. Vertical bars indicate mean ± SD (n = 3). * Significant difference at p < 0.05.
