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Figure 3. Viral pre-infection time and bacterial adherence. (A) Effect of hCMV pre-infection time on adherence of Actinobacillus actinomycetemcomitans strains to HeLa cells. Cells were exposed for 1 hr to hCMV (MOI 100) and incubated for an additional 6, 12, 24, or 48 hrs, or were mock-infected for 48 hrs (squares). Uninfected cells served as controls. Data are expressed as the proportion of cell-associated bacteria relative to the numbers of bacteria added to the cells initially. Each datapoint represents the mean ± SEM for 12 independent experiments. *P < 0.05, hCMV-infected group vs. uninfected control; #P < 0.05, 48-hour hCMV-infected group vs. 48-hour mock-infected group; °P < 0.05, 48-hour vs. 24-hour hCMV-infected group. (B) Effect of hCMV pre-infection time on adherence of Actinobacillus actinomycetemcomitans strain JP2 to primary cells. Cells were exposed for 1 hr to hCMV or mock preparation (MOI 100) and incubated for an additional 6, 12, 24, or 48 hrs. Uninfected cells served as controls. Data are expressed as the proportion of cell-associated bacteria relative to the numbers of bacteria added to the cells initially. Each datapoint represents the mean ± SEM for 8 independent experiments. *P < 0.05, hCMV- or mock-infected group vs. uninfected control; #P < 0.05, hCMV vs. mock-infected group.
