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Figure 4. Ameloblastin cleavage sites in vivo and in vitro. Analysis of porcine ameloblastin cleavage products isolated from developing teeth show that ameloblastin is cleaved in vivo after Asn31 (a), Gln130 (b), Arg170 (c), Ala222 (d), Gly300 (e), Arg319 (f), and Ala342 (g). Three AMBN cleavage sites targeted by MMP-20 in vitro are identical to known in vivo cleavage sites (after Gln130, Arg170, and A222). Two sites that were cleaved in vitro have not been identified in vivo: Pro2 (h*) and Gln139 (i*). The context of the Gln139 site (Q:QV) is identical to the Gln130 site, suggesting that this site is likely to be cleaved in vivo, but in vivo cleavage products starting with this cleavage have not yet been described. Key: The in vivo and in vitro AMBN cleavage sites are marked by arrows. The arrows are boxed for the sites cleaved in vitro.
