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Figure 1. Purified rpAMBN expressed in 293F mammalian cells and activated rpMMP-20. (A) The rpAMBN migrated as a smeared band at 65 kDa on 4–12% NuPage gels. The rAMBN reacted with Coomassie brilliant blue (CBB), stains-all (SA), and a glycoprotein gel stain (Pro-Q). The rpAMBN reacted with 3 antibodies: 2 AMBN anti-peptide antibodies (AMBN-63 and AMBN-381) and a monoclonal antibody specific for the V5 epitope (V5) fused to the AMBN C-terminus. (B) The 25-kDa rpMMP-20 catalytic domain used to digest rpAMBN is indicated by an arrowhead on a casein zymogram (Zym).
