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Figure 2. Immunochemical localization of various glycosaminoglycans (GAGs): chondroitin sulfate for A, B, C; keratan sulfate for D, E; heparan sulfate for F, G; dermatan sulfate for H, I. The bone-marrow-derived osteoblastic cells were cultured for 28 days on the titanium-coated polystyrene (A,B,D,F,H) or cell-culture-grade polystyrene (C,E,G,I). Ti, titanium; P, polystyrene. See text for the details of the histological preparation, immunostaining, and titanium coating. Bar is 20 µm.
