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Figure 1. Expression of bone-related proteoglycan genes in the bone-marrow-derived osteoblastic cultures analyzed by reverse-transcriptase/polymerase chain-reaction (RT-PCR). The osteoblastic cells were cultured on either a polystyrene dish, a machined titanium surface, or an acid-etched titanium surface. (A) A representative PCR analysis, at multiple time-points of culture, visualized on a 1.5% agarose gel with ethidium bromide staining. (B) The expression level at multiple time-points normalized to GAPDH mRNA expression.
