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Figure 4. Hematoxylin and eosin staining demonstrating in vivo bone formation. Transplants of untreated control periosteal cells (-) formed little bone (a). Transplants of periosteal cells that received BMP-2 treatment alone (BMP) (b) or received FGF pre-treatment alone (pre-treatment) (c) also formed new bone. Periosteal cells that were treated with BMP-2 after FGF pre-treatment (pre-treatment + BMP) formed significant amounts of new bone (d). Bar indicates 300 µm. Arrow indicates new bone. The difference in new bone volume between periosteal cells (black) and marrow stromal cells (white) was determined by histomorphometric analysis (e). Asterisk indicates significant difference (p < 0.05) between paired conditions. Values are means ± standard deviation for 3 sections of each sample.
