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Figure 2. Periosteal cells (black) and marrow stromal cells (white) were plated in 12-well plates at a density of 1.0 x 105 cells/well. Then, the cells were incubated with bFGF (a) or BMP-2 (b) for 6 days. Alkaline phosphatase activity was analyzed on day 7 of culture. In (a), asterisk indicates significant difference (p < 0.05), compared with the control (–). FGF-pre-treated periosteal cells are indicated by black hatched bars; non-pre-treated periosteal cells are indicated by black bars; FGF-pre-treated marrow stromal cells are indicated by white hatched bars; non-pre-treated marrow stromal cells are indicated by white bars (c,d). Values are means ± standard deviation for 3 cultures. In b–d, asterisk indicates significant difference (p < 0.05) between paired conditions.
