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Figure 4. Effect of inhibiting ERK phosphorylation on Emdogain-induced increase in human gingival fibroblast cell numbers and DNA synthesis. (A) Cells were pre-treated with U0126 (0, 0.5, 5 µM) before the addition of Emdogain (EMD) in the presence of 0.5% serum and stained with crystal violet. Dye uptake without Emdogain and U0126 (= control) was set to 100%. (B) Thymidine incorporation of human gingival fibroblasts after pre-treatment with different concentrations of U0126. (C) Effects of U0126 (5 µM) on Emdogain-induced DNA synthesis, when the inhibitor was added 1 hr before or 3 hrs after the addition of Emdogain. Each bar represents the mean ± SD of 3–6 wells. (D) Effect of Emdogain on collagen production by 3H proline incorporation. *p < 0.05, **p < 0.005.
