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Figure 3. Western blot analysis of the effects of Emdogain (EMD) and U0126 on ERK1/2 phosphorylation in human gingival fibroblasts. (A) Quiescent cells were stimulated with Emdogain in serum-free medium for either 15–60 min or 4–18 hrs. (B) Dose-dependent inhibition of Emdogain-induced ERK phosphorylation by U0126. Lower bands show the abundance of total ERK as loading control.

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