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Figure 2. Effects of Emdogain (EMD) and fetal calf serum on human gingival fibroblast proliferation. (A) Quiescent cells were stimulated with Emdogain and fetal calf serum (0 and 0.5%) for 24 hrs. [3H] thymidine was added for the last 4 hrs. Each bar represents the mean ± SD of 3–6 wells. (B) Representative histogram plots of flow cytometric analysis. Quiescent cells were stimulated with Emdogain for 25 hrs in serum-free conditions and stained with propidium iodide (PI) for DNA content. (C) Summary of cell-cycle kinetics of human gingival fibroblasts (% of total) in the presence of 0 or 0.5% fetal calf serum ± Emdogain, 25 hrs following stimulation. Each bar represents the mean ± SD of 3–6 wells. (D) Western blot analysis of cyclin D1 and cyclin B1, 12 and 24 hrs, respectively, after quiescent cells were treated with Emdogain. Lower bands show the abundance of ß-actin in these samples for loading control. In A and C, *p < 0.05, **p < 0.005, Emdogain 50 vs. Emdogain 0 µg/mL.
