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Figure 4. Results of microdissection analysis of BDNF mRNA and protein expression in rats immobilization-stressed for 60 min. (A) BDNF-reactivity was found within duct cells, but not acinar cells, by immunofluorescence staining. Scale bar = 10 µm. (B) Duct cells and acinar cells dissected from tissue sections. Scale bar = 10 µm. (C) Cap image showing dissected duct cells. Scale bar = 20 µm. (D) Cap image showing dissected acinar cells. Scale bar = 20 µm. (E) Graph showing BDNF/ß-actin ratios of the dissected samples. BDNF mRNA level was 0.0001 for duct cells, and 0 for acinar cells. Duct cells showed the presence of BDNF mRNA, but acinar cells did not.
