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Figure 3. BDNF protein levels following immobilization stress. (A) Photomicrographs showing the immunohistochemical localization of BDNF protein in paraffin-embedded tissues from rats immobilization-stressed with the anti-BDNF monoclonal antibody (n = 6). BDNF protein expression was observed in duct cells. Scale bar = 10 µm. (B) Western blotting demonstrated bands of 14 kDa in brain tissue and submandibular gland tissues of the immobilization-stress group (n = 6). P, Positive control (brain tissue).