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Figure 1. BDNF mRNA quantification in rat submandibular gland tissue. (A) Graph showing BDNF mRNA of immobilization-stressed rats. BDNF mRNA level was 0.000367 ± 0.000205 for non-stress, 0.009028 ± 0.001 for 30 min, 0.001021 ± 0.000474 for 60 min, or 0.001463 ± 0.000662 for 180 min of immobilization stress (n = 6, bar = SD). There were significant differences between non-stress and 30, 60, or 180 min (*p < 0.01). (B) Graph showing BDNF mRNA levels in the post-immobilization stress (rats exposed to 30 min of immobilization stress were immediately killed at 30, 60, or 180 min after the termination of restraint). BDNF mRNA level was 0.001181 ± 0.000137 at 30 min, 0.000973 ± 0.000075 at 60 min, or 0.000135 ± 0.000031 at 180 min (n = 6, bar = SD). There was a significant difference between non-stress and 30 or 60 min of post-immobilization stress (*p < 0.01). BDNF mRNA levels approached those of non-stressed rats at 180 min of post-immobilization stress.
