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Figure 1. Desipramine inhibits intracellular pH recovery in acidified human submandibular acinar cells. (A) 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-loaded dissociated human submandibular acinar cells were perfused with normal HCO3– buffers and then challenged with 15 mM NH4Cl (hatched bar) for acid loading. Thereafter, the bath solution was changed to a Na+-free HCO3–buffer (blank bar). After stabilization of the pH level, the intracellular pH recovery patterns were monitored in the absence or presence of 300 nM desipramine (filled bar). Typical pH traces from more than 10 separate experiments are presented. (B) Concentration-dependent effects of desipramine on pH recovery. Cells were treated with various concentrations of desipramine after acid-loading, and the rate of pH recovery was monitored. Each point was obtained from more than 3 separate experiments and is the mean ± SEM. The results were reproducible.
