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Figure 3. Effect of ala42S100A8 on lipopolysaccharide (LPS)-induced recruitment of neutrophils in a rat air-pouch model. The data represent a mean (± SD). (A) Air-pouches were injected with 300 µL of a PBS control, 30 ng LPS in PBS, and 30 ng LPS in PBS containing 10–5 M of ala42S100A8. Co-injection of ala42S100A8 inhibited the inflammatory response induced by LPS, as indicated by the number of neutrophils recruited by LPS into the air-pouch (*p < 0.01 when compared with PBS-only control). (B) Dose response of the inhibitory effect of ala42S100A8 on the recruitment of neutrophils induced by LPS in the air-pouch model. All the air-pouches were injected with 30 ng LPS in 300 µL PBS containing different concentrations of ala42S100A8. ala42S100A8 displayed dose-dependent inhibition of the recruitment of neutrophils (#p < 0.05; *p < 0.01 when compared with LPS-only control; n indicates the number of rats in each treatment group). PMN, polymorphonuclear leukocyte.
