Click on image to view larger version.
Figure 4. Expression of FGF-2 and VEGF in human pulp cells. Pulp fibroblasts were cultured in four-well culture chambers. Injuries were made with scalpels on confluent cultures. After a five-hour culture period, immunohistochemistry showed the expression of FGF-2 (A) and VEGF (B) at a low level in pulp fibroblasts. Stronger expression of FGF-2 (D) and VEGF (E) could be observed after injury. Controls with no primary antibodies were negative in intact (C) and injured cells (F). Arrows indicate injury sites. Scale bars = 100 µm.
