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Figure 4. Pulp cell differentiation in the presence of amelogenins as measured by alkaline phosphatase (ALP) and dentin sialoprotein (DSP) expression. In dental papilla cells, ALP mRNA expression (A) or ALP protein synthesis (B) was not altered in the presence of 10 nM rH58 (lane 1), rH72 (lane 2), or rH174 (lane 3). Control with no added amelogenins (lane 4). In dental pulp cells, ALP mRNA and DSP expression (C) or protein synthesis (D) was similarly not altered in the presence of 10 nM rH58 (lane 1), rH72 (lane 2), or rH174 (lane 3). Control with no added amelogenins (lane 4). p > 0.05.
