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Figure 4. RT-PCR and immunohistochemistry of the TREK-1 K+ channel in cultured PDL fibroblasts. (A) Analysis of the expression of TREK-1 by RT-PCR. Lane 1 contains the PCR product of TREK-1. The RT-PCR product migrates in the gel to a position in good agreement with its predicted size of 492 bp. Lane 2 contains PCR-amplified product of TREK-2. Lane 3 shows RT-PCR product of GAPDH (571 bp). GAPDH was amplified to verify equal loading of RNA. Lane 4 shows the DNA molecular size standard. The results show that cultured human PDL fibroblasts do express TREK-1, but not TREK-2. (B) Immunocytochemical staining of TREK-1 K+ channels in cultured human PDL fibroblasts. PDL fibroblasts were stained with goat anti-human TREK-1 polyclonal antibody (TREK-1) or IgG as a negative control (IgG). White bar, 20 µm.
