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Figure 4. Anti-RANKL IgG or OPG blocks OA-synovial fluid-induced osteoclast formation. The reconstituted synovial fluid (OA) was applied to the osteoclast formation assay using PBMC, and to the pit-formation assay, in the presence of M-CSF (100 ng/mL), along with anti-human RANKL IgG (10 µg/mL, PeproTeck, London, UK) or human recombinant OPG (200 ng/mL, R&D Systems). ***P < 0.005 compared with the data obtained in the absence of either additive. Experiments were repeated 3 times with similar results. Arrows point to osteoclasts. Original magnification, 40x for TRAP staining (bar = 200 µm), and 100x for pit-formation assay (Bar = 500 µm).
