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Figure 3. Time (5 and 10 min)-dependent effects of bradykinin on the t-Akt/PKB, p-Akt/PKB, t-ERK1/2, and p-ERK1/2 in odontoblasts. In odontoblasts treated with BK for 5 (I, control, 52.78 ± 13.42/BK, 83.55 ± 22.42) and 10 (J, control [A], 113.35 ± 5.30/BK [B], 110.06 ± 9.15) min, there was no significant difference in the staining intensities for total (t)-Akt/PKB between control and treated odontoblasts. Localization of phosphorylated (p)-Akt/PKB was not identifiable in the control odontoblasts, while the signal intensities were increased after 5 (I, control, 33.54 ± 10.18/BK, 156.75 ± 6.90; asterisks) and 10 (J, control [C], 35.95 ± 5.66/BK [D], 77.38 ± 4.63; asterisks) min of BK treatment. 5 (I, control, 159.80 ± 7.81/BK, 154.98 ± 3.53) and 10 (J, control [E], 150.57 ± 2.14/BK [F], 157.72 ± 2.99) min of BK treatment did not affect staining intensities of t-ERK1/2 in either control or treated odontoblasts. The staining intensities for p-ERK1/2 were not detectable in control (G) or in odontoblasts treated with BK after 5 (I, control, 28.47 ± 2.68/BK, 34.46 ± 11.19) or 10 (J, control [G], 17.72 ± 1.97/BK [H], 29.15 ± 7.33) min. Data are mean ± SD; n = 4 for each group. Significant differences were considered at a P value < 0.05. Bar = 50 µm.
