Click on image to view larger version.
Figure 2. Diabetes impaired new bone formation and increased osteoblastic apoptosis. The area of new bone formation (A) was measured in TRAP-stained sections as described in MATERIALS & METHODS. The area of alveolar bone between the cemento-enamel junction (CEJ) and a line 1 mm apical to the CEJ was measured in H&E-stained sections (B). Rats described in Fig. 1 were examined for the percent of apoptotic bone-lining cells by the TUNEL assay (C) and the number of bone-lining cells per mm bone length in H&E-stained sections (D). Each value is the mean of 5 to 7 rats ± SE. *Significant difference between diabetics and normoglycemic control rats (P < 0.05).
