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Figure 1. Diabetes prolongs osteoclast formation and activity. Ligatures were placed around the molar teeth of ZDF type 2 diabetic and normoglycemic control rats and left in place for 7 days. Rats were killed prior to the placement of ligatures (no ligatures), immediately after removal (0 day), or 4 or 9 days later. Histologic TRAP-stained sections, original magnification 200x. Large open arrows indicate osteoclasts, large closed arrows indicate Howship’s lacunae, small closed arrows point to a reversal line, and small open arrows point to the extent of new bone formation (A). Osteoclasts were counted (B), and the percent eroded bone surface (C) was measured in TRAP-stained sections. Each value in B and C is the mean of 5 to 7 rats ± SE. *Significant difference between diabetics and normoglycemic control rats (P < 0.05). Bar in far left panel represents 0.05 mm.
