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Figure 2. High-magnification views taken from the peritubular regions of sclerotic dentin after immunolabeling. (A) Labeling for collagen fibrils (black arrows) is sparse in this region. A few clusters of 15-nm nanoparticles (open arrowheads) that were bound to the anti-chondroitin sulfate monoclonal antibodies can also be identified. (B) Another specimen showing sparse labeling for antigenically intact type I collagen. Labeling for proteoglycans cannot be observed.