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Figure 1. Western blotting analyses and immunostaining of the rat submandibular gland on the injection side (inj) following injection of rCLCA siRNA (A,C) or CFTR siRNA (B,D), and from the submandibular gland on the non-injection side (no inj). Corresponding antibodies were used (anti-rCLCA for A and C, anti-CFTR for B and D). (A,B) Intensity of the immunoreactive bands (90 kDa for A and 170 kDa for B) is summarized in bar graphs. Means ± SEM. *P < 0.05; **P < 0.01 vs. corresponding "no inj" (paired t test, N = 5 for A and N = 6 for B). Protein loading was 25 µg per lane. (C,D) Bar = 50 µm. ST, striated duct; G, granular convoluted tubule; AC, acini.
