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Figure 3. Example of a cross-section of a fiber bundle from the anterior belly of the digastric muscle (A-D) and the electrophoresis results (E,F). (B-D) Magnifications of the indicated area in A after incubation with antibodies against MyHC-I (B), MyHC-IIA (C), and MyHC-IIA+IIX (D). Bar = 100 µm. (E) MyHC composition of this bundle as determined by gel electrophoresis. Only the MyHCs are shown. The plot obtained from laser densitometry (F) shows the integrated optical density of this particular fiber bundle.