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Figure 2. Neighbor-joining tree showing phylogeny of 16S rDNA sequences obtained directly from cases of primary endodontic infection. Sequences were aligned with ClustalW, and distances were calculated with the Jukes-Cantor algorithm. Five hundred bootstrap trees were generated, and bootstrap confidence levels as percentages (only values over 50%) are shown at tree nodes. Accession numbers for each 16S rDNA sequence are given. Scale bar shows number of nucleotide substitutions per site.