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Figure 1. Biological activity induced by unfractionated Emd-Gel. Mv1Lu cells were transfected with PAI-1 gene, then treated with (A) TGF-ß1 (0.05, 0.1, 0.2, 0.4, 0.6 ng/mL) and (B) Emd-Gel (10, 20, 30, 40, 50 µg/mL). C2C12 cells were transfected with Id1 gene, and then treated with (C) BMP2 (50, 100, 150, 200, 250 ng/mL) and (D) Emd-Gel (25, 50, 75, 100, 125 µg/mL). Luciferase activity was determined after an overnight incubation. Data are means ± SE of 4 culture wells. *p < 0.05, **p < 0.001.
