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Figure 1. TNF or LPS activates IKK. (A) DPSCs were treated with TNF (10 ng/mL) and P. gingivalis LPS (1 µg/mL) for the indicated times. Fifty-µg aliquots of protein extracts were probed with polyclonal antibodies against I
B
(1:1000) or monoclonal antibodies against phospho-specific antibodies (1:1000). For loading control, the blots were stripped and re-examined with monoclonal antibodies against -tubulin (1:15, 000). (B) E. coli LPS activates IKK. DPSCs were treated with E. coli LPS (200 ng/mL) or TNF for the indicated time points. Western blot analysis was performed as described in (A).
