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RESEARCH REPORT |
B Activation in Human Dental Pulp Stem Cells by TNF and LPS
1 Laboratory of Molecular Signaling and Apoptosis, Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA;
2 School of Stomatology, Peking University Health Science Center, Beijing, China;
3 Division of Operative Dentistry and Endodontics, Department of Oral Medicine, Kanagawa Dental College, Kanagawa, Japan; and
4 Section of Oral Biology, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA;
* corresponding author, cunywang{at}umich.edu
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS & METHODSRESULTSDISCUSSIONREFERENCES |
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B) in DPSCs. We found that both TNF and LPS activated the I-kappa B kinase complex (IKK) in DPSCs to induce the phosphorylation and degradation of IB
, resulting in the nuclear translocation of NF-B. Consistently, both TNF and LPS rapidly induced the expression of the NF-B-dependent gene interleukin-8 (IL-8). However, unlike in monocytes, we found that LPS could not induce the phosphorylation of the NF-B active subunit p65 in DPSCs. In summary, our studies suggest that DPSCs may be involved in immune responses during pulpal infection through activating NF-B.
KEY WORDS: NF-B • dental pulp stem cells • tumor necrosis factor • LPS • inflammation.
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS & METHODSRESULTSDISCUSSIONREFERENCES |
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The dental pulp tissues from which DPSCs are derived are frequently infected or inflamed due to bacterial infection from dental caries (Stashenko et al., 1998). LPS, a major component of the outer membrane of bacteria, has been identified in infected pulp tissues (Wang et al., 1997; Rupf et al., 2000; Darveau et al., 2002). Pro-inflammatory cytokines, such as TNF and IL-1, have been found to be highly expressed in inflamed pulp tissues (Stashenko et al., 1998; Coil et al., 2004). Importantly, elegant studies by Rutherford and Gu (2000) demonstrated that local dentin/pulp inflammation interfered with odontoblast differentiation and dentin repair. While monocytes or fibroblasts in dental pulp tissues have been found to express pro-inflammatory cytokines, it is not clear whether DPSCs are involved in host response and/or produce inflammatory mediators upon bacterial infection.
It is well-known that LPS stimulates monocytes, lymphocytes, and certain types of fibroblasts to produce pro-inflammatory cytokines, such as TNF and IL-1, in chronic oral inflammatory diseases such as pulpitis, and periapical and periodontal diseases (Nagaoka et al., 1996; Wang et al., 1997; Kawashima and Stashenko, 1999; Kent et al., 1999; Fouad and Acosta, 2001). Elevated levels of TNF and IL-1 can stimulate a variety of cell types to generate inflammatory mediators and chemokines to amplify inflammatory responses (Liu et al., 1996; Baqui et al., 1998). It is believed that the critical factor activated by LPS and TNF during infection is the transcription factor NF-B (Baldwin, 2001). Genes known to be regulated by NF-B include major histocompatibility molecules (MHC), IL-1ß, IL2, IL-2 receptor, IL-6, IL-8, intercellular adhesion molecule-1, E-selectin, TNF, and interferon-
. In the unstimulated condition, NF-B is present in an inactive form that is retained in the cytoplasm by the inhibitory protein IB. The most common form of NF-B is a heterodimer of p50 and p65/RelA proteins. Biochemical and genetic studies have found that the IKK complex plays a critical role in the activation of NF-B. The IKK complex consists mainly of two catalytic subunits, IKK and IKKß, and a non-catalytic chaperone protein IKK (Baldwin, 2001). Recently, we have found that the zinc-finger structure of IKK also plays a critical role in IKK activation (Yang et al., 2004). Upon stimulation by LPS, TNF, or other stimuli, IKK and IKKß are activated following IKK ubiquitination by unknown mechanisms. The activated IKK complex phosphorylates the N-terminal region of IB at Serines 32 and 36. The phosphorylated IB is subsequently ubiquitinated and degraded by the 26S proteasome machinery. The degradation of IB releases NF-B from cytoplasm to the nucleus, where NF-B binds to specific elements in the promoter of NF-B target genes and stimulates gene transcription (Wang et al., 1996, 1998; Baldwin, 2001). In this study, to understand the role of DPSCs in pulpal infection and host response, we examined whether LPS or TNF activated the NF-B signaling pathway in DPSC cells.
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MATERIALS & METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS & METHODSRESULTSDISCUSSIONREFERENCES |
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Western Blot Analysis
DPSCs were treated with TNF (10 ng/mL) or LPS (from 200 ng to 1 µg/mL) for different time periods. Cells underwent lysis in RIPA buffer (10 mM Tris-HCl, 1 mM EDTA, 1% sodium dodecyl sulfate [SDS], 1% Nonidet P-40, 1:100 proteinase inhibitor cocktail, 50 mM ß-glycerophosphate, 50 mM sodium fluoride). Fifty-µg aliquots of protein extracts were subjected to 10% SDS-polyacrylamide gel electrophoresis and transferred to PVDF membrane by a semi-dry transfer apparatus (Bio-Rad, Hercules, CA, USA). Western blot analysis was performed as described previously (Yang et al., 2003, 2004). Primary antibodies were purchased from the following commercial sources: monoclonal antibodies against human IB (1:1000; Santa Cruz, CA, USA); monoclonal antibodies against phospho-specific IB and polyclonal antibodies against phospho-specific p65 (Cell Signaling, Beverly, MA, USA); and monoclonal antibodies against -tubulin (Sigma).
Northern Blot Analysis
DPSCs were treated with TNF or LPS, and total RNAs were extracted with Trizol reagents according to the manufacturer’s instructions (Invitrogen). Ten-µg aliquots of total RNAs were separated on a 1.4% agarose-formaldehyde gel, transferred onto a nylon membrane (Bio-Rad), and cross-linked with a UV cross-linker. IL-8 or IL-6 probes were generated with a random-primed labeling kit (Amersham Biosciences, Piscataway, NJ, USA) in the presence of [-32P]dCTP (MP Biomedicals, Irvine, CA, USA), and purified via a micro-G-50 Sephadex column (Amersham Biosciences). The hybridization was performed as described previously (You et al., 2001).
Electrophoretic Mobility Shift Assays (EMSAs)
DPSCs were treated with TNF (10 ng/mL) or P. gingivalis LPS (1 µg/mL), pelleted, and washed twice with ice-cold PBS. Nuclear protein isolation and EMSAs were performed as described previously (Yang et al., 2004). For EMSAs, 5-µg aliquots of nuclear extracts were incubated for 15 min at room temperature with 2 x 104 cpm of a 32P-labeled oligonucleotide probe containing a B site (underlined) from the class I MHC promoter (5'-CAGGGCTGGGGATTCCCCATCTCCACAGTTTCACTTC-3' in nuclear binding buffer plus 2 µg of poly (dI-dC) (Pharmacia Biotech, Piscataway, NJ, USA). For the super-shift assay to confirm NF-B-binding specificity, we added 1 µL of rabbit polyclonal antibodies against p65 subunit (Rockland) of NF-B to nuclear extracts for 15 min prior to the addition of poly(dI-dC)-poly(dI-dC) and a 32P-labeled oligonucleotide probe, and then separated them on 5% polyacrylamide gels (Wang et al., 1999a,b).
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS & METHODSRESULTSDISCUSSIONREFERENCES |
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B signaling pathway was intact in DPSCs, we first examined whether TNF induced the phosphorylation and degradation of IB by activating IKK. After TNF treatment, whole-cell extracts were prepared, and Western blot analysis was performed with monoclonal antibodies against phospho-specific IB (Yang et al., 2003, 2004). The phosphorylation of IB in DPSCs was rapidly induced upon TNF stimulation in 5 min (Fig. 1A
). Subsequently, the degradation of IB by 26S proteasome occurred in DPSCs. Because IB was degraded, we were unable to detect the phosphorylated IB at 30-minute time points. However, due to the fact that the promoter of IB is also regulated by NF-B, IB was rapidly re-synthesized after TNF treatment. Therefore, the phosphorylated IB was detected again at 60- and 120-minute time points (Fig. 1A). The induction of IB by NF-B serves as a feedback mechanism for negative regulation of NF-B activation.
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B in monocytes and macrophages (Yang et al., 2003, 2004). Compared with TNF, P. gingivalis LPS induced modest phosphorylation of IB (Fig. 1A, 
top panel). However, it appeared that the level of IB phosphorylation was sufficient to trigger the degradation of IB in DPSCs (Fig. 1A, middle panel). To determine whether LPS from other bacteria also activated NF-B in DPSCs, we utilized E. coli LPS. Like P. gingivalis LPS, E. coli LPS also induced the phosphorylation and degradation of IB in DPSCs (Fig. 1B). Taken together, our results suggest that both TNF and LPS induce IKK activity in DPSCs.
After IB degradation, it is believed that NF-B is moved to the nucleus to bind to the promoter region of NF-B target genes and to stimulate transcription. To determine whether TNF induced nuclear translocation of NF-B in DPSCs, we isolated the nuclear proteins from DPSCs and subjected them to electrophoretic mobility shift assays (EMSAs), using 32P-labeled NF-B oligonucleotide probes. TNF rapidly induced the nuclear translocation of NF-B in DPSCs (Fig. 2A). To confirm the specificity of NF-B-binding activity, we pre-incubated the nuclear proteins from TNF-treated DPSCs with anti-p65 antibodies for 10 min and then incubated them with NF-B oligonucleotide probes. The pre-treatment of anti-p65 antibodies supershifted NF-B-binding activity in DPSCs (Fig. 2A, compare lane 3 with lane 6), confirming the specificity of the NF-B-binding activity. Similarly, the nuclear translocation of NF-B in DPSCs was also induced by P. gingivalis LPS (Fig. 2B) and E. coli LPS (data not shown).
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B subunit p65 was phosphorylated by the IKK complex in monocytes or macrophages upon stimulation of TNF or P. gingivalis LPS. The phosphorylation of p65 was required for optimal activation of NF-B-dependent gene transcription in monocytes or macrophages (Yang et al., 2003). Thus, we also examined whether TNF or LPS induced phosphorylation of p65 in DPSCs. On the contrary, we found that TNF weakly induced the phosphorylation of p65 on serine 536, as detected by phospho-specific p65 antibodies (Fig. 3A). Moreover, we did not find that P. gingivalis LPS induced the phosphorylation of p65 on serine 536 in DPSCs. Our previous studies found that the phosphorylation of p65 had effects on NF-B transcription in monocytes or macrophages. Since TNF or LPS weakly induced, or could not induce, p65 phosphorylation, we were interested in determining whether TNF or LPS functionally stimulated NF-B-dependent transcription. However, because DPSCs were resistant to transfection, we were unable to utilize NF-B-dependent luciferase reporter assays for our studies. Since it is well-known that the expression of IL-8 is dependent on NF-B activation, we performed Northern blot analysis to determine whether TNF or LPS induced IL-8 expression through activating NF-B. Both TNF and P. gingivalis LPS rapidly induced the expression of IL-8 expression, as detected by Northern blot analysis (Figs. 3B, 3C), suggesting that they activated NF-B transcription in DPSCs. To confirm our results, we examined whether the lack of p65 phosphorylation affected the expression of IL-6, another NF-B-dependent pro-inflammatory cytokine. We found IL-6 was also rapidly induced by both TNF and E. coli LPS in DPSCs (Figs. 3D, 3E).
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B in DPSCs. P. endodontalis LPS also stimulated the IKK complex to phosphorylate IB. The level of IB was decreased following stimulation, suggesting that IB was partially degraded (Fig. 4A). Consistently, we found that P. endodontalis induced the nuclear translocation of NF-B. However, P. endodontalis LPS did not induce the phosphorylation of p65 (Fig. 4B).
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS & METHODSRESULTSDISCUSSIONREFERENCES |
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B signaling pathway in DPSCs. NF-B has been considered as a master transcription factor that regulates a variety of inflammatory mediators, including TNF, IL-1, IL-6, and IL-8 (Baldwin, 2001). Previously, it has been reported that LPS or TNF can induce the production of pro-inflammatory cytokines in dental pulp fibroblasts, cementum-like fibroblasts, and oral epithelial cells (Nakane et al., 1995; Salvi et al., 1997; Chiang et al., 1999; Martin et al., 2001; Ogawa et al., 2002; Nociti et al., 2004). However, the underlying molecular mechanisms which control the expression of these inflammatory mediators in these cells have not been explored. Our results are the first demonstration that NF-B is functionally activated by both TNF and LPS in DPSCs. Also, according to our results, the activation of NF-B by TNF or LPS might be responsible for the production of pro-inflammatory cytokines in dental pulp fibroblasts and other cells. NF-B may be an important target for inhibiting oral and dental inflammation.
While the NF-B subunit p50 has been found to bind to DNA, the NF-B subunit p65 has transactivation domains which are critical for NF-B transcription (Baldwin, 2001). Recently, we have found that the IKK complex activated by TNF and P. gingivalis LPS also phosphorylated the p65 trans-activation domain on serine 536 in human monocytes or mouse macrophages. The phosphorylation of p65 potentiated NF-B transactivation (Yang et al., 2003). In contrast, we found that LPS could not induce phosphorylation of p65, while TNF only weakly stimulated p65 phosphorylation in DPSCs. Currently, we cannot provide an explanation for this difference. It is possible that the conformation of the IKK complex in DPSCs may be different from that in monocytes or macrophages. Although the activated IKK complex could sufficiently phosphorylate IB, it might be unable to phosphorylate p65 in DPSCs. Alternatively, it is also possible that the trans-activation domain of p65 might be masked by other molecules in DPSCs which interfere with the phosphorylation of p65 by the IKK complex. Nevertheless, we found that both LPS and TNF could rapidly induce the expression of the NF-B-dependent gene IL-8, suggesting that the phosphorylation of p65 may not be required for NF-B transcription in DPSCs. Finally, it should be mentioned that the phosphorylation of p65 may be required for the induction of a specific group of NF-B target genes. It is possible that these genes may be induced by TNF or LPS in monocytes or macrophages, but not in DPSCs. The identification of these genes may further help us to understand the biological properties of DPSCs.
Interestingly, we also observed that TNF and LPS exhibited different patterns of NF-B activation. NF-B-binding activities were induced by LPS in 30 min and disappeared by 60 min. In contrast, NF-B-binding activities were further increased after 60 min following TNF stimulation. This difference may be due to the fact that TNF induced the phosphorylation and degradation of IB more potently than did LPS in DPSCs. However, since TNF and LPS utilized different receptor pathways to activate IKK, NF-B-binding activities may be regulated at the second level by different components associated with the TNF or LPS intracellular signaling pathways. In the future, it will be interesting to compare the gene expression profiles induced by TNF and LPS in DPSCs.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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J.C. and C.Z. contributed equally to this work; 
Received January 27, 2005; Last revision July 14, 2005; Accepted July 19, 2005
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