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Figure 2. Time-course of NaF-induced apoptosis in HL-60 cells. HL-60 cells (5 x 105/mL) were incubated for the indicated times with 0, 7.5, or 10 mM NaF to assay caspase-3, -8, and -9 activity (A), internucleosomal DNA fragmentation (B), and the number of apoptotic cells (C), respectively. (A) Mean ± SD (n = 3), 10 mM NaF, 1 µg/mL actinomycin D (Act.D.) (positive control). (B) Representative of 3 independent experiments, 7.5 mM NaF. (C) Mean ± SE (n = 4), 0, 7.5, or 10 mM NaF. At least 200 cells were observed under by light microscopy. **p < 0.001, *p < 0.01, 
p < 0.05.
