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Figure 1. Dose-response of NaF-induced apoptosis in HL-60 cells. HL-60 cells (5 x 105/mL) were incubated for 4 (A), 6 (B), 24 (C), or 6 (D) hrs with the indicated concentrations of NaF to assay caspase-3, -8, and -9 activity (A), internucleosomal DNA fragmentation (B), the viable cell number (C), and the dysfunction of mitochondrial membrane potential in NaF-treated HL-60 cells (D), respectively. (A) Mean ± SD (n = 3). (B) Representative of 3 independent experiments. (C) Mean ± SE (n = 6). At least 400 cells were observed under a light microscope. (D) Representative of 3 independent experiments. Right panel shows the quantification of fluorescence intensity (FI) of apoptotic cells/viable cells. **p < 0.001, *p < 0.01, 
p < 0.05.
