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Figure 3. Effects of rhIL-1
and PGE2 on the expression of RANKL, M-CSF, and OPG mRNAs in odontogenic keratocyst fibroblasts. (A) Effects of rhIL-1 on the expression of RANKL mRNA in odontogenic keratocyst fibroblasts. Odontogenic keratocyst fibroblasts were incubated in serum-free DMEM for 12 hrs at 37°C in the absence or presence of rhIL-1. RT-PCR amplifications were performed at 35 cycles for RANKL and 27 cycles for ß-actin, respectively, as described in MATERIALS & METHODS. (B) Effects of PGE2 on the expression of RANKL, M-CSF, and OPG mRNAs in odontogenic keratocyst fibroblasts. Odontogenic keratocyst fibroblasts were incubated in serum-free DMEM for 12 hrs at 37°C in the absence (lane 1) or presence of 10 nM rhIL-1 (lanes 2 and 3) or 10 µM PGE2 (lane 4). Indomethacin (1 µM) was added 15 min before the application of 10 nM rhIL-1 (lane 3). RT-PCR amplifications were performed at 35 cycles for RANKL, 30 cycles for M-CSF, 25 cycles for OPG, and 27 cycles for ß-actin, respectively, as described in MATERIALS & METHODS.
