Click on image to view larger version.
Figure 1. Effects of positive pressure on the expression of IL-1
and on the plasma membrane permeability. (A) Odontogenic keratocyst epithelial cells were incubated in serum-free DMEM for 15 min at 37°C under atmospheric pressure or 80 mm Hg of positive pressure. Total cellular RNA was extracted, and RT-PCR amplifications were performed at 30 cycles for IL-1, and 27 cycles for ß-actin, as described in MATERIALS & METHODS. (B) Odontogenic keratocyst epithelial cells were cultured in serum-free DMEM for 24 hrs at 37°C under atmospheric pressure or 80 mm Hg of positive pressure. The concentration of IL-1 in the conditioned media was measured by ELISA, as described in MATERIALS & METHODS. Vertical bars indicate mean ± SD (n = 4). *Significant difference between atmospheric pressure and positive pressure at p < 0.05. (C) Odontogenic keratocyst epithelial cells were incubated with 5 µM fluo-3 AM for 30 min at room temperature, as described in MATERIALS & METHODS. The fluorescent intensity for fluo-3 was monitored before (a) and after (b) application of 80 mm Hg positive pressure to the cells. Bar represents 40 µm.
