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Figure 3. Presence of IL-4 and IL-10 in peritoneal exudates from mice injected with DSP (A) and DSP-stimulated mast cell supernatant (B). Peritoneal exudates of mice injected with DSP (1 µg/mL) (
) or PBS (
) were recovered after 6 hrs. MC (5 x 105 cells/well) were treated with DSP (10 µg/mL) and washed, and supernatant was recovered after 1 hr. Cytokine production was assessed by ELISA. Results are mean ± SEM of 10 mice per group. The experiment was repeated twice. *P < 0.05 compared with PBS.