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Figure 1. (A) Role of mast cells (MC) and (B) macrophages in DSP- and DPP-induced neutrophil migration. Resident MC were depleted, and the macrophage population was augmented by treatment of mice with 48/80 compound (
) (A) and thioglycolate () (B), respectively, as described in MATERIALS & METHODS. Control mice were pre-treated with PBS (
). After pre-treatment, mice were injected i.p. with PBS (C), fMLP (100 nmol/cavity), DSP, and DPP (1 µg/mL), and neutrophil migration was assessed 6 hrs later. Results are mean ± SEM of 10 mice per group. The experiment was repeated twice. *P < 0.05 compared with respective control; #P < 0.05 compared with groups pre-treated with PBS.
