Click on image to view larger version.

Figure 2. Folin phenol assay of epigallocatechin (egc). (A) A standard curve of increasing amounts of epigallocatechin up to 25 µg in solution. (B) Increasing amounts of epigallocatechin (up to 25 µg) were incubated with 5 mg of hydroxyapatite, and the bound epigallocatechin was assayed. Approximately 0.25 µg of epigallocatechin bound to hydroxyapatite from an aqueous solution containing 50 µg of epigallocatechin. Values are means ± standard deviation of 6 assays.